Anti-Human CD31/PECAM1 Recombinant Antibody (SAA2179) (HB004033)

Name: Anti-Human CD31/PECAM1 Recombinant Antibody (SAA2179)
Brand: abinScience
SKU: HB004033
Price: 110 USD
Availability: InStock

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Спецификация:

20ug
50ug
100ug
1mg

Количество:

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Обзор
Изображения
Ссылки
Даташит
SDS

Обзор

Каталожный номер

HB004033

Реактивность видов

Human

Применения

ELISA, WB, FCM

Хозяинский вид

Mouse

Клоональность

Monoclonal

Изотип

VHH-mFc (IgG2a)

ID клона

SAA2179

Проверенные приложения

FCM

Цель

Platelet endothelial cell adhesion molecule, PECAM-1, EndoCAM, GPIIA', PECA1, CD31, PECAM1

Уровень эндотоксинов

Please contact with the lab for this information.

Чистота

>95% as determined by SDS-PAGE.

Очистка

Protein A/G purified from cell culture supernatant.

Номер доступа

P16284

RRID

Anti-Human CD31/PECAM1 Antibody (SAA2179) (abinScience Cat# HB004033, RRID:AB_3729544)

Форма

Liquid

Буфер хранения

0.01M PBS pH 7.4

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта

Применение	Разбавление
ELISA	1:2000-1:20000
WB	1:500-1:2000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Фон

Platelet endothelial cell adhesion molecule (CD31/PECAM1) is a ~82 kDa protein. Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions. Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils. Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal.

1. Sachs, UJ. et al. (2007) The Journal of biological chemistry 282, 23603-12. PMID: 17580308

2. Dasgupta, B. et al. (2009) Journal of immunology (Baltimore, Md. : 1950) 182, 5041-51. PMID: 19342684

3. Hu, M. et al. (2016) Scientific reports 6, 38655. PMID: 27958302

4. Brown, S. et al. (2002) Nature 418, 200-3. PMID: 12110892

5. Yeh, JC. et al. (2008) Biochemistry 47, 9029-39. PMID: 18672896

Примечание

For research use only.

Isotype Control

Mouse IgG2a, kappa Recombinant Isotype Control Antibody (MOPC-173) (MF533507)

Изображения

Flow cytometry

Flow-cytometry using anti-human CD31 antibody. Jurkat cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD31 monoclonal antibody (Catalog HB004033, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human CD31 antibody. THP-1 cells were stained with an irrelevant antibody (Green Histogram) or an anti-human CD31 antibody monoclonal antibody (Catalog # HB004033 , Blue Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (AntibodySystem: MF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE

SDS-PAGE for Anti-Human CD31/PECAM1 Antibody (SAA2179)