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Anti-Human GITR/TNFRSF18 Recombinant Antibody (hu6C8) (HV475207)

Anti-Human GITR/TNFRSF18 Recombinant Antibody (hu6C8)
Anti-Human GITR/TNFRSF18 Recombinant Antibody (hu6C8)
Anti-Human GITR/TNFRSF18 Recombinant Antibody (hu6C8)
Anti-Human GITR/TNFRSF18 Recombinant Antibody (hu6C8)
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Aperçu
Numéro de catalogueHV475207
Reactivité des espècesHuman
ApplicationsFCM
Espèce hôteHuman
ClonalitéMonoclonal
IsotypeIgG1, kappa
ID de clonehu6C8
Applications testées FCM
Cible Activation-inducible TNFR family receptor, GITR, TNFRSF18, CD357, Tumor necrosis factor receptor superfamily member 18, AITR, Glucocorticoid-induced TNFR-related protein
Niveau d'endotoxines Please contact with the lab for this information.
Pureté >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Numéro d'accès Q9Y5U5
Forme Liquid
Tampon de stockage 0.01M PBS pH 7.4

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Contexte

Tumor necrosis factor receptor superfamily member 18 (CD357/GITR) is a ~26 kDa protein. Receptor for TNFSF18. Seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. Mediated NF-kappa-B activation via the TRAF2/NIK pathway.

Note For research use only.
Images
  • Anti-Human GITR/TNFRSF18 Recombinant Antibody (hu6C8)

    Bioactivity

    SEC-HPLC detection for Anti-Human CD357/TNFRSF18/GITR Antibody (hu6C8).

  • Anti-Human GITR/TNFRSF18 Recombinant Antibody (hu6C8)

    Flow Cytometry

    Flow-cytometry using anti-human CD357 antibody. Human RPMI-8226 cell line were stained with an irrelevant antibody (Green Histogram) or an anti-human CD357 antibody monoclonal antibody (Catalog # HV475207 , Red Histogram) at a concentration of 20 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.

Reférences
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Recommandation

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