Anti-Human GITR Ligand/TNFSF18 Reference Antibody (Ragifilimab, RUO) (HV475016)

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100ug
1mg

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.	HV475016
Description	Ragifilimab (HV475016) is a research-grade recombinant antibody targeting CD357. Produced in mammalian cells with native-like glycosylation. Highlights ●Research Grade — For PK/PD studies, assay development, and ADA research. ●Native Glycosylation — Mammalian expression ensures native-like patterns.
Species reactivity	Human
Applications	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Host species	Humanized
Isotype	IgG1-kappa
Expression system	Mammalian Cells
Clonality	Monoclonal
Target	Activation-inducible TNFR family receptor, GITR, TNFRSF18, CD357, Tumor necrosis factor receptor superfamily member 18, AITR, Glucocorticoid-induced TNFR-related protein
Endotoxin level	Please contact the lab for this information.
Purity	>95% purity as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	Q9Y5U5
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background	Tumor necrosis factor receptor superfamily member 18 (CD357/GITR) is a ~26 kDa protein. Receptor for TNFSF18. Seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. Mediated NF-kappa-B activation via the TRAF2/NIK pathway.
Note	For research use only. Not suitable for clinical or therapeutic use.

Images

Bioactivity

Detects CD357/TNFRSF18/GITR in indirect ELISAs.
Flow CytoMetry

Flow-cytometry using APC anti-human CD357 antibody. RPMI-8226 cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD357 monoclonal antibody (Catalog: HV475016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using PE anti-human CD357 antibody. RPMI-8226 cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD357 monoclonal antibody (Catalog: HV475016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Western blot

Western blot analysis was performed using anti-CD357/TNFRSF18/GITR monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human CD357/TNFRSF18/GITR (Catalog No: HV475011)
Lane 2: negative control
SDS-PAGE

SDS-PAGE for Research Grade Ragifilimab