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Anti-Human Integrin alpha 2/CD49b Recombinant Antibody (SAA0018), PE (HB772127)

Anti-Human Integrin alpha 2/CD49b Recombinant Antibody (SAA0018), PE
Anti-Human Integrin alpha 2/CD49b Recombinant Antibody (SAA0018), PE
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  • 50T
  • 100T
Nombre :
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Aperçu
Numéro de catalogueHB772127
Reactivité des espècesHuman
ApplicationsFCM
Espèce hôteMouse
ClonalitéMonoclonal
IsotypeIgG2a, kappa
ID de cloneSAA0018
ConjugaisonPE
Cible Collagen receptor, ITGA2, VLA-2 subunit alpha, CD49B, CD49 antigen-like family member B, Platelet membrane glycoprotein Ia, GPIa, Integrin alpha-2, CD49b
Niveau d'endotoxines < 10 EU/mg
Pureté >90% as determined by SDS-PAGE.
Produits associés Mouse IgG2a kappa Recombinant kappa Recombinant Isotype Control Antibody (MOPC-173), PE
Numéro d'accès P17301
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Stabilité et stockage Store at 4°C for 12 months. Protect from light. Do not freeze.
Contexte

Integrin alpha-2 (CD49b/ITGA2) is a ~129 kDa protein. Integrin alpha-2/beta-1 is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix.

Note For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.
Images
  • Anti-Human Integrin alpha 2/CD49b Recombinant Antibody (SAA0018), PE

    Flow CytoMetry

    Flow-cytometry using PE anti-human CD49b antibody. HUVEC cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD49b monoclonal antibody (Catalog: HB772127, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

Reférences
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Recommandation

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