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Anti-Human Integrin alpha 2/CD49b Recombinant Antibody (SAA0018) (HB772107)

Anti-Human Integrin alpha 2/CD49b Recombinant Antibody (SAA0018)
Anti-Human Integrin alpha 2/CD49b Recombinant Antibody (SAA0018)
Anti-Human Integrin alpha 2/CD49b Recombinant Antibody (SAA0018)
Anti-Human Integrin alpha 2/CD49b Recombinant Antibody (SAA0018)
Anti-Human Integrin alpha 2/CD49b Recombinant Antibody (SAA0018)
Anti-Human Integrin alpha 2/CD49b Recombinant Antibody (SAA0018)
Anti-Human Integrin alpha 2/CD49b Recombinant Antibody (SAA0018)
Anti-Human Integrin alpha 2/CD49b Recombinant Antibody (SAA0018)
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Overview
Catalog No.HB772107
Species reactivityHuman
ApplicationsFCM
Host speciesMouse
IsotypeIgG2a, kappa
Clone IDSAA0018
Clonality Monoclonal
Target Collagen receptor, ITGA2, VLA-2 subunit alpha, CD49B, CD49 antigen-like family member B, Platelet membrane glycoprotein Ia, GPIa, Integrin alpha-2, CD49b
Endotoxin level Please contact with the lab for this information.
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Accession P17301
Form Liquid
Storage buffer 0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background

Integrin alpha-2 (CD49b/ITGA2) is a ~129 kDa protein. Integrin alpha-2/beta-1 is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix.

Note For research use only.
Images
  • Anti-Human Integrin alpha 2/CD49b Recombinant Antibody (SAA0018)

    Flow Cytometry

    Flow-cytometry using anti-human CD49b antibody.293T cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD49b antibody monoclonal antibody (Catalog # HB772107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

  • Anti-Human Integrin alpha 2/CD49b Recombinant Antibody (SAA0018)

    Flow Cytometry

    Flow-cytometry using anti-human CD49b antibody.HaCaT cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD49b antibody monoclonal antibody (Catalog # HB772107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

  • Anti-Human Integrin alpha 2/CD49b Recombinant Antibody (SAA0018)

    Flow Cytometry

    Flow-cytometry using anti-human CD49b antibody.HUVEC cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD49b antibody monoclonal antibody (Catalog # HB772107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

  • Anti-Human Integrin alpha 2/CD49b Recombinant Antibody (SAA0018)

    Flow Cytometry

    Flow-cytometry using anti-human CD49b antibody.A431 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD49b antibody monoclonal antibody (Catalog # HB772107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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