



| Número de catálogo | HY302207 |
|---|---|
| Reactividad de especies | Human |
| Aplicaciones | FCM |
| Especie huésped | Mouse |
| Clonalidad | Monoclonal |
| Isotipo | IgG2b, kappa |
| ID de clon | 3A1e |
| Aplicaciones probadas | FCM |
| Objetivo | GP40, CD7, T-cell surface antigen Leu-9, T-cell leukemia antigen, TP41, T-cell antigen CD7 |
| Nivel de endotoxinas | Please contact with the lab for this information. |
| Pureza | >95% as determined by SDS-PAGE. |
| Purificación | Protein A/G purified from cell culture supernatant. |
| Número de acceso | P09564 |
| Forma | Liquid |
| Buffer de almacenamiento | 0.01M PBS pH 7.4 Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote. |
| Estabilidad y almacenamiento | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| Fondo | T-cell antigen CD7 is a ~25 kDa protein. Transmembrane glycoprotein expressed by T-cells and natural killer (NK) cells and their precursors. Plays a costimulatory role in T-cell activation upon binding to its ligand K12/SECTM1. In turn, mediates the production of cytokines such as IL-2. On resting NK-cells, CD7 activation results in a significant induction of interferon-gamma levels. 1. Rabinowich, H. et al. (1994) Journal of immunology (Baltimore, Md. : 1950) 152, 517-26. PMID: 7506726 2. Lyman, SD. et al. (2000) The Journal of biological chemistry 275, 3431-7. PMID: 10652336 3. Carrel, S. et al. (1991) European journal of immunology 21, 1195-200. PMID: 1709867 |
| Nota | For research use only. |

SEC-HPLC detection for Anti-Human CD7 Antibody (3A1e).

Flow-cytometry using anti-human CD7 antibody. Human Jurkat cell line were stained with an irrelevant antibody (Green Histogram) or an anti-human CD7 antibody monoclonal antibody (Catalog # HY302207 , Red Histogram) at a concentration of 5µg/ml for 30 mins at RT. After washing, bound antibody was detected using a CoraLite488 conjugated goat anti-mouse antibody (Catalog # MF690314) and cells analysed on a NovoCyte Flow Cytometer.




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