Anti-Human CD7 Recombinant Antibody (3A1e) (HY302207)

Name: Anti-Human CD7 Recombinant Antibody (3A1e)
Brand: abinScience
SKU: HY302207
Price: 163 USD
Availability: InStock

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概要
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参考文献
データシート
SDS

概要

カタログ番号	HY302207
種反応性	Human
アプリケーション	FCM
宿主種	Mouse
クローン性	Monoclonal
アイソタイプ	IgG2b, kappa
クローンID	3A1e
テスト済みアプリケーション	FCM
ターゲット	GP40, CD7, T-cell surface antigen Leu-9, T-cell leukemia antigen, TP41, T-cell antigen CD7
エンドトキシンレベル	Please contact with the lab for this information.
純度	>95% as determined by SDS-PAGE.
精製	Protein A/G purified from cell culture supernatant.
アクセッション番号	P09564
形態	Liquid
保存バッファー	0.01M PBS pH 7.4 データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。
安定性と保存	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
背景	T-cell antigen CD7 is a ~25 kDa protein. Transmembrane glycoprotein expressed by T-cells and natural killer (NK) cells and their precursors. Plays a costimulatory role in T-cell activation upon binding to its ligand K12/SECTM1. In turn, mediates the production of cytokines such as IL-2. On resting NK-cells, CD7 activation results in a significant induction of interferon-gamma levels. 1. Rabinowich, H. et al. (1994) Journal of immunology (Baltimore, Md. : 1950) 152, 517-26. PMID: 7506726 2. Lyman, SD. et al. (2000) The Journal of biological chemistry 275, 3431-7. PMID: 10652336 3. Carrel, S. et al. (1991) European journal of immunology 21, 1195-200. PMID: 1709867
注意事項	For research use only.

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Bioactivity

SEC-HPLC detection for Anti-Human CD7 Antibody (3A1e).
Flow Cytometry

Flow-cytometry using anti-human CD7 antibody. Human Jurkat cell line were stained with an irrelevant antibody (Green Histogram) or an anti-human CD7 antibody monoclonal antibody (Catalog # HY302207 , Red Histogram) at a concentration of 5µg/ml for 30 mins at RT. After washing, bound antibody was detected using a CoraLite488 conjugated goat anti-mouse antibody (Catalog # MF690314) and cells analysed on a NovoCyte Flow Cytometer.