



| Número de catálogo | HF815207 |
|---|---|
| Reactividad de especies | Human |
| Aplicaciones | ELISA, FCM, IP |
| Especie huésped | Mouse |
| Clonalidad | Monoclonal |
| Isotipo | IgG2a kappa |
| ID de clon | OKT8 |
| Aplicaciones probadas | FCM |
| Objetivo | CD8a, Cd8a, Lyt-2, Lyt2, T-cell surface glycoprotein CD8 alpha chain, T-cell surface glycoprotein Lyt-2 |
| Nivel de endotoxinas | < 10 EU/mg |
| Pureza | >95% as determined by SDS-PAGE. |
| Purificación | Protein A/G purified from cell culture supernatant. |
| Número de acceso | P01732 |
| Forma | Liquid |
| Buffer de almacenamiento | 0.01M PBS pH 7.4. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote. |
| Estabilidad y almacenamiento | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Fondo | T-cell surface glycoprotein CD8 alpha chain (CD8a/Lyt2) is a ~25 kDa protein. Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. |
| Nota | For research use only. |

Flow-cytometry using anti-human CD8a antibody.CD8a Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD8a antibody monoclonal antibody (Catalog # HF815207 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

SDS-PAGE for Anti-Human CD8a/Lyt2 Antibody (OKT8)




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