Anti-Human CD8 alpha Recombinant Antibody (mab1) (HF815107)

Name: Anti-Human CD8 alpha Recombinant Antibody (mab1)
Brand: abinScience
SKU: HF815107
Price: 163 USD
Availability: InStock

Datasheet Share product Copy info

Price(USD): --

Spec:

50ug
100ug

Number:

Get quote

Overview
Images
References
Datasheet
SDS

Overview

Catalog No.	HF815107
Description	Anti-Human CD8 alpha Recombinant Antibody (mab1) (HF815107) is a mouse monoclonal antibody detecting CD8 alpha in FCM. Suitable for Human. Highlights ●Affinity Purified — Minimal background and high purity for reliable results.
Species reactivity	Human
Applications	FCM
Host species	Mouse
Clonality	Monoclonal
Isotype	IgG1, kappa
Clone ID	mab1
Tested applications	FCM
Target	CD8a, Cd8a, Lyt-2, Lyt2, T-cell surface glycoprotein CD8 alpha chain, T-cell surface glycoprotein Lyt-2
Endotoxin level	< 10 EU/mg as determined by LAL method.
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	P01732
Form	Liquid
Storage buffer	0.01M PBS pH 7.4. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background	T-cell surface glycoprotein CD8 alpha chain (CD8a/Lyt2) is a ~25 kDa protein. Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex.
Note	For research use only.

Images

Flow-cytometry

Flow-cytometry using anti- human CD8 antibody.
Human RPMI-8226 cell line were stained with an irrelevant antibody (Gray Histogram) or an anti- human CD8 antibody monoclonal antibody (Catalog # HF815107 , Red Histogram) at a concentration of 20ug/ml for 30 mins at RT. After washing, bound antibody was detected using a CoraLite488 conjugated goat anti-mouse antibody (Catalog # MF690314) and cells analysed on a NovoCyte Flow Cytometer.
Bioactivity

SEC-HPLC detection for Anti-Human CD8A Antibody (mab1).

References

Datasheet

Anti-Human CD8 alpha Recombinant Antibody (mab1).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human CD8 alpha Recombinant Antibody (mab1) (HF815107)

Overview

Images

References

Datasheet

SDS