Anti-Human CD8 alpha Recombinant Antibody (OKT8) (HF815207)

Name: Anti-Human CD8 alpha Recombinant Antibody (OKT8)
Brand: abinScience
SKU: HF815207
Price: 196 USD
Availability: InStock

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Aperçu

Numéro de catalogue	HF815207
Reactivité des espèces	Human
Applications	ELISA, FCM, IP
Espèce hôte	Mouse
Clonalité	Monoclonal
Isotype	IgG2a kappa
ID de clone	OKT8
Applications testées	FCM
Cible	CD8a, Cd8a, Lyt-2, Lyt2, T-cell surface glycoprotein CD8 alpha chain, T-cell surface glycoprotein Lyt-2
Niveau d'endotoxines	< 10 EU/mg
Pureté	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Numéro d'accès	P01732
Forme	Liquid
Tampon de stockage	0.01M PBS pH 7.4. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Stabilité et stockage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte	T-cell surface glycoprotein CD8 alpha chain (CD8a/Lyt2) is a ~25 kDa protein. Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex.
Note	For research use only.

Images

Flow-cytometry

Flow-cytometry using anti-human CD8a antibody.CD8a Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD8a antibody monoclonal antibody (Catalog # HF815207 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE

SDS-PAGE for Anti-Human CD8a/Lyt2 Antibody (OKT8)