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Anti-Human CD8 alpha Recombinant Antibody (OKT8) (HF815207)

Anti-Human CD8 alpha Recombinant Antibody (OKT8)
Anti-Human CD8 alpha Recombinant Antibody (OKT8)
Anti-Human CD8 alpha Recombinant Antibody (OKT8)
Anti-Human CD8 alpha Recombinant Antibody (OKT8)
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  • 概要

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  • 参考文献

  • データシート

  • SDS

概要
カタログ番号HF815207
種反応性Human
アプリケーションELISA, FCM, IP
宿主種Mouse
クローン性Monoclonal
アイソタイプIgG2a kappa
クローンIDOKT8
テスト済みアプリケーション FCM
ターゲット CD8a, Cd8a, Lyt-2, Lyt2, T-cell surface glycoprotein CD8 alpha chain, T-cell surface glycoprotein Lyt-2
エンドトキシンレベル < 10 EU/mg
純度 >95% as determined by SDS-PAGE.
精製 Protein A/G purified from cell culture supernatant.
アクセッション番号 P01732
形態 Liquid
保存バッファー 0.01M PBS pH 7.4.

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

安定性と保存 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

T-cell surface glycoprotein CD8 alpha chain (CD8a/Lyt2) is a ~25 kDa protein. Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex.

注意事項 For research use only.
イメージ
  • Anti-Human CD8 alpha Recombinant Antibody (OKT8)

    Flow-cytometry

    Flow-cytometry using anti-human CD8a antibody.CD8a Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD8a antibody monoclonal antibody (Catalog # HF815207 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

  • Anti-Human CD8 alpha Recombinant Antibody (OKT8)

    SDS-PAGE

    SDS-PAGE for Anti-Human CD8a/Lyt2 Antibody (OKT8)

参考文献
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
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Volume
Molecular Weight *
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Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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