Anti-Human DNMT3A IP Recombinant Antibody (SAA0199) (HV233013)

Name: Anti-Human DNMT3A IP Recombinant Antibody (SAA0199)
Brand: abinScience
SKU: HV233013
Price: 126 USD
Availability: InStock

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Спецификация:

20ug
50ug
100ug
1mg

Количество:

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Обзор
Изображения
Ссылки
Даташит
SDS

Обзор

Каталожный номер

HV233013

Реактивность видов

Human

Применения

ELISA, FCM, IF, IP

Хозяинский вид

Mouse

Клоональность

Monoclonal

Изотип

IgG2a

ID клона

SAA0199

Цель

Dnmt3a, DNA MTase HsaIIIA, DNMT3A, M.HsaIIIA, DNA (cytosine-5)-methyltransferase 3A, DNA methyltransferase HsaIIIA

Уровень эндотоксинов

Please contact with the lab for this information.

Чистота

>95% as determined by SDS-PAGE.

Очистка

Protein A/G purified from cell culture supernatant.

Номер доступа

Q9Y6K1

RRID

Anti-Human DNMT3A IP Antibody (SAA0199) (abinScience Cat# HV233013, RRID:AB_3729733)

Форма

Liquid

Буфер хранения

0.01M PBS pH 7.4

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта

Применение	Разбавление
ELISA	1:2000-1:20000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Фон

DNA (cytosine-5)-methyltransferase 3A (DNMT3A) is a ~101 kDa protein. Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting.

1. Chen, T. et al. (2002) The Journal of biological chemistry 277, 38746-54. PMID: 12138111

2. Viré, E. et al. (2006) Nature 439, 871-4. PMID: 16357870

3. Heyn, P. et al. (2019) Nature genetics 51, 96-105. PMID: 30478443

Примечание

For research use only.

Isotype Control

Mouse IgG2a (Fc Silence) Recombinant Isotype Control (MOPC 173) (MF533023)

Изображения

Flow Cytometry

Flow-cytometry using anti-human DNMT3A antibody.293T cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human DNMT3A antibody monoclonal antibody (Catalog # HV233013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human DNMT3A antibody.HepG2 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human DNMT3A antibody monoclonal antibody (Catalog # HV233013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human DNMT3A antibody.Human Jurkat cell line were stained with an irrelevant antibody (Blue Histogram) or an anti-human DNMT3A antibody monoclonal antibody (Catalog # HV233013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human DNMT3A antibody.HeLa cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human DNMT3A antibody monoclonal antibody (Catalog # HV233013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE

SDS-PAGE for Anti-Human DNMT3A IP Antibody (SAA0199).
Flow CytoMetry

Flow-cytometry using FITC anti-human DNMT3A antibody. HepG-2 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human DNMT3A monoclonal antibody (Catalog: HV233013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using PE anti-human DNMT3A antibody. HepG-2 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an PE anti-human DNMT3A monoclonal antibody (Catalog: HV233013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

Ссылки

Даташит

Anti-Human DNMT3A IP Recombinant Antibody (SAA0199).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Рекомбинантные Белки

Антитела

Наборы для анализа