Anti-DNMT3A Polyclonal Antibody (HV233014)

Name: Anti-DNMT3A Polyclonal Antibody
Brand: abinScience
SKU: HV233014
Price: 168 USD
Availability: InStock

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SDS

Overview

Catalog No.

HV233014

Description

Anti-DNMT3A Polyclonal Antibody (HV233014) is a rabbit polyclonal antibody detecting Dnmt3a in ELISA, IHC, WB. Suitable for Chicken, Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human DNMT3A (Ala628-Val912).

Target

Dnmt3a, DNA MTase HsaIIIA, DNMT3A, M.HsaIIIA, DNA (cytosine-5)-methyltransferase 3A, DNA methyltransferase HsaIIIA

Purification

Purified by antigen affinity column.

Accession

Q9Y6K1

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

DNA (cytosine-5)-methyltransferase 3A (DNMT3A) is a ~101 kDa protein. Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting.

1. Chen, T. et al. (2002) The Journal of biological chemistry 277, 38746-54. PMID: 12138111

2. Viré, E. et al. (2006) Nature 439, 871-4. PMID: 16357870

3. Heyn, P. et al. (2019) Nature genetics 51, 96-105. PMID: 30478443

Note

For research use only.

Images

References

Datasheet

Anti-DNMT3A Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-DNMT3A Polyclonal Antibody (HV233014)

Overview

Images

References

Datasheet

SDS

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