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Anti-Human Fibronectin/EDB Recombinant Antibody (SAA1375) (HY259023)

Anti-Human Fibronectin/EDB Recombinant Antibody (SAA1375)
Anti-Human Fibronectin/EDB Recombinant Antibody (SAA1375)
Anti-Human Fibronectin/EDB Recombinant Antibody (SAA1375)
Anti-Human Fibronectin/EDB Recombinant Antibody (SAA1375)
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  • 1mg
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Aperçu
Numéro de catalogueHY259023
Reactivité des espècesHuman
ApplicationsBlocking, ELISA, IHC, WB
Espèce hôteMouse
ClonalitéMonoclonal
IsotypeIgG2a, kappa
ID de cloneSAA1375
Cible Cold-insoluble globulin, CIG, FN, Fibronectin, FN1, EDB, EDB-FN
Niveau d'endotoxines Please contact with the lab for this information.
Pureté >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Numéro d'accès P02751
RRID

Anti-Human EDB/EDB-FN Antibody (SAA1375) (abinScience Cat# HY259023, RRID:AB_3726892)
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Forme Liquid
Tampon de stockage 0.01M PBS pH 7.4

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit
Application Dilution
ELISA 1:2000-1:20000
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Contexte

Fibronectin (EDB) is a ~272 kDa protein. Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts. Acts as a ligand for the LILRB4 receptor, inhibiting FCGR1A/CD64-mediated monocyte activation.

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1. Owens, RJ. et al. (1986) The EMBO journal 5, 2825-30. PMID: 3024962
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2. Garcia-Pardo, A. et al. (1987) The Biochemical journal 241, 923-8. PMID: 3593230
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3. Calaycay, J. et al. (1985) The Journal of biological chemistry 260, 12136-41. PMID: 3900070
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4. Rostagno, A. et al. (1994) The Journal of biological chemistry 269, 31938-45. PMID: 7989369
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5. Su, MT. et al. (2021) International immunology 33, 447-458. PMID: 34089617
Note For research use only.
Images
  • Anti-Human Fibronectin/EDB Recombinant Antibody (SAA1375)

    SDS-PAGE

    SDS-PAGE for Anti-Human EDB/EDB-FN Recombinant Antibody (SAA1375)

  • Anti-Human Fibronectin/EDB Recombinant Antibody (SAA1375)

    Bioactivity

    Detects EDB EDB-FN (Catlog No: HY259022) in indirect ELISA.

Reférences
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Recommandation

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