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Anti-Human FGF-19 Recombinant Antibody (SAA1435) (HF862013)

Anti-Human FGF-19 Recombinant Antibody (SAA1435)
Anti-Human FGF-19 Recombinant Antibody (SAA1435)
Anti-Human FGF-19 Recombinant Antibody (SAA1435)
Anti-Human FGF-19 Recombinant Antibody (SAA1435)
Anti-Human FGF-19 Recombinant Antibody (SAA1435)
Anti-Human FGF-19 Recombinant Antibody (SAA1435)
Anti-Human FGF-19 Recombinant Antibody (SAA1435)
Anti-Human FGF-19 Recombinant Antibody (SAA1435)
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Especificación:
  • 20ug
  • 50ug
  • 100ug
  • 1mg
Número:
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Vista general
Número de catálogoHF862013
Reactividad de especiesHuman
AplicacionesELISA, FCM, IHC, WB
Especie huéspedHuman
ClonalidadMonoclonal
IsotipoIgG1
ID de clonSAA1435
Objetivo FGF19, Fibroblast growth factor 19, FGF-19
Nivel de endotoxinas Please contact with the lab for this information.
Pureza >95% as determined by SDS-PAGE.
Purificación Protein A/G purified from cell culture supernatant.
Número de acceso O95750
Forma Liquid
Buffer de almacenamiento 0.01M PBS pH 7.4

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto
Aplicación Dilución
ELISA 1:2000-1:20000
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Fondo

Fibroblast growth factor 19 (FGF19) is a ~24 kDa protein. Involved in the suppression of bile acid biosynthesis through down-regulation of CYP7A1 expression, following positive regulation of the JNK and ERK1/2 cascades. Stimulates glucose uptake in adipocytes. Activity requires the presence of KLB and FGFR4.

Nota For research use only.
Isotype Control Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507)
Imágenes
  • Anti-Human FGF-19 Recombinant Antibody (SAA1435)

    Bioactivity

    SEC-HPLC detection for Anti-Human FGF19 Antibody (ABS0484).

  • Anti-Human FGF-19 Recombinant Antibody (SAA1435)

    Flow Cytometry

    Flow-cytometry using anti-human FGF19 antibody.HT-29 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human FGF19 antibody monoclonal antibody (Catalog # HF862013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.

  • Anti-Human FGF-19 Recombinant Antibody (SAA1435)

    SDS-PAGE

    SDS-PAGE for Anti-Human FGF19 Antibody (SAA1435).

  • Anti-Human FGF-19 Recombinant Antibody (SAA1435)

    Western blot

    Western blot analysis was performed using anti-human FGF19 monoclonal antibody at 1ug/mL on various samples.
    Lane 1: recombinant human FGF19 (Catalog No: HF862012)
    Lane 2: negative control

Referencias
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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