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Anti-Human FGF-19 Recombinant Antibody (SAA1435) (HF862013)

Anti-Human FGF-19 Recombinant Antibody (SAA1435)
Anti-Human FGF-19 Recombinant Antibody (SAA1435)
Anti-Human FGF-19 Recombinant Antibody (SAA1435)
Anti-Human FGF-19 Recombinant Antibody (SAA1435)
Anti-Human FGF-19 Recombinant Antibody (SAA1435)
Anti-Human FGF-19 Recombinant Antibody (SAA1435)
Anti-Human FGF-19 Recombinant Antibody (SAA1435)
Anti-Human FGF-19 Recombinant Antibody (SAA1435)
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Aperçu
Numéro de catalogueHF862013
Reactivité des espècesHuman
ApplicationsELISA, FCM, IHC, WB
Espèce hôteHuman
ClonalitéMonoclonal
IsotypeIgG1
ID de cloneSAA1435
Cible FGF19, Fibroblast growth factor 19, FGF-19
Niveau d'endotoxines Please contact with the lab for this information.
Pureté >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Numéro d'accès O95750
Forme Liquid
Tampon de stockage 0.01M PBS pH 7.4

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit
Application Dilution
ELISA 1:2000-1:20000
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Contexte

Fibroblast growth factor 19 (FGF19) is a ~24 kDa protein. Involved in the suppression of bile acid biosynthesis through down-regulation of CYP7A1 expression, following positive regulation of the JNK and ERK1/2 cascades. Stimulates glucose uptake in adipocytes. Activity requires the presence of KLB and FGFR4.

Note For research use only.
Isotype Control Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507)
Images
  • Anti-Human FGF-19 Recombinant Antibody (SAA1435)

    Bioactivity

    SEC-HPLC detection for Anti-Human FGF19 Antibody (ABS0484).

  • Anti-Human FGF-19 Recombinant Antibody (SAA1435)

    Flow Cytometry

    Flow-cytometry using anti-human FGF19 antibody.HT-29 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human FGF19 antibody monoclonal antibody (Catalog # HF862013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.

  • Anti-Human FGF-19 Recombinant Antibody (SAA1435)

    SDS-PAGE

    SDS-PAGE for Anti-Human FGF19 Antibody (SAA1435).

  • Anti-Human FGF-19 Recombinant Antibody (SAA1435)

    Western blot

    Western blot analysis was performed using anti-human FGF19 monoclonal antibody at 1ug/mL on various samples.
    Lane 1: recombinant human FGF19 (Catalog No: HF862012)
    Lane 2: negative control

Reférences
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Recommandation

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