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| カタログ番号 | HF862013 | ||||||
|---|---|---|---|---|---|---|---|
| 種反応性 | Human | ||||||
| アプリケーション | ELISA, FCM, IHC, WB | ||||||
| 宿主種 | Human | ||||||
| クローン性 | Monoclonal | ||||||
| アイソタイプ | IgG1 | ||||||
| クローンID | SAA1435 | ||||||
| ターゲット | FGF19, Fibroblast growth factor 19, FGF-19 | ||||||
| エンドトキシンレベル | Please contact with the lab for this information. | ||||||
| 純度 | >95% as determined by SDS-PAGE. | ||||||
| 精製 | Protein A/G purified from cell culture supernatant. | ||||||
| アクセッション番号 | O95750 | ||||||
| 形態 | Liquid | ||||||
| 保存バッファー | 0.01M PBS pH 7.4 データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. | ||||||
| 背景 | Fibroblast growth factor 19 (FGF19) is a ~24 kDa protein. Involved in the suppression of bile acid biosynthesis through down-regulation of CYP7A1 expression, following positive regulation of the JNK and ERK1/2 cascades. Stimulates glucose uptake in adipocytes. Activity requires the presence of KLB and FGFR4. | ||||||
| 注意事項 | For research use only. | ||||||
| Isotype Control | Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507) |
SEC-HPLC detection for Anti-Human FGF19 Antibody (ABS0484).
Flow-cytometry using anti-human FGF19 antibody.HT-29 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human FGF19 antibody monoclonal antibody (Catalog # HF862013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE for Anti-Human FGF19 Antibody (SAA1435).
Western blot analysis was performed using anti-human FGF19 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human FGF19 (Catalog No: HF862012)
Lane 2: negative control
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