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Anti-Human Fibronectin Recombinant Antibody (SAA0522) (HY259013)

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Anti-Human Fibronectin Recombinant Antibody (SAA0522)
Anti-Human Fibronectin Recombinant Antibody (SAA0522)
Anti-Human Fibronectin Recombinant Antibody (SAA0522)
Anti-Human Fibronectin Recombinant Antibody (SAA0522)
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  • 1mg
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Vista general
Número de catálogoHY259013
Descripción
Anti-Human Fibronectin Recombinant Antibody (SAA0522) (HY259013) is a mouse monoclonal antibody detecting Fibronectin in ELISA, IF, IHC, SPR, WB, FCM. Suitable for Human, Mouse, Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactividad de especiesHuman, Mouse, Rat
AplicacionesELISA, IF, IHC, SPR, WB, FCM
Especie huéspedMouse
ClonalidadMonoclonal
IsotipoIgG2a, kappa
ID de clonSAA0522
Aplicaciones probadas FCM
Objetivo Cold-insoluble globulin, CIG, FN, Fibronectin, FN1
Nivel de endotoxinas < 10 EU/mg as determined by LAL method.
Pureza >95% as determined by SDS-PAGE.
Purificación Protein A/G purified from cell culture supernatant.
Número de acceso P02751
RRID Anti-Human FN1/Fibronectin Antibody (SAA0522) (abinScience Cat# HY259013, RRID:AB_3726039)
Forma Liquid
Buffer de almacenamiento 0.01M PBS pH 7.4

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Información de uso del producto
Aplicación Dilución
ELISA 1:2000-1:20000
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Fondo

Fibronectin (EDB) is a ~272 kDa protein. Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts. Acts as a ligand for the LILRB4 receptor, inhibiting FCGR1A/CD64-mediated monocyte activation.

1. Owens, RJ. et al. (1986) The EMBO journal 5, 2825-30. PMID: 3024962
2. Garcia-Pardo, A. et al. (1987) The Biochemical journal 241, 923-8. PMID: 3593230
3. Calaycay, J. et al. (1985) The Journal of biological chemistry 260, 12136-41. PMID: 3900070
4. Rostagno, A. et al. (1994) The Journal of biological chemistry 269, 31938-45. PMID: 7989369
5. Su, MT. et al. (2021) International immunology 33, 447-458. PMID: 34089617
Nota For research use only.
Isotype Control Mouse IgG2a, kappa Recombinant Isotype Control Antibody (MOPC-173) (MF533507)
Imágenes
  • Anti-Human Fibronectin Recombinant Antibody (SAA0522)

    SDS-PAGE

    SDS-PAGE for Anti-Human FN1/Fibronectin Antibody (SAA0522)

  • Anti-Human Fibronectin Recombinant Antibody (SAA0522)

    Flow cytometry

    Flow-cytometry using anti-human FN1 antibody. HepG2 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human FN1 monoclonal antibody (Catalog HY259013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.

Referencias
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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