Anti-Human Fibronectin Recombinant Antibody (SAA0522) (HY259013)

Name: Anti-Human Fibronectin Recombinant Antibody (SAA0522)
Brand: abinScience
SKU: HY259013
Price: 126 USD
Availability: InStock

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20ug
50ug
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1mg

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Aperçu

Numéro de catalogue

HY259013

Description

Anti-Human Fibronectin Recombinant Antibody (SAA0522) (HY259013) is a mouse monoclonal antibody detecting Fibronectin in ELISA, IF, IHC, SPR, WB, FCM. Suitable for Human, Mouse, Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactivité des espèces

Human, Mouse, Rat

Applications

ELISA, IF, IHC, SPR, WB, FCM

Espèce hôte

Mouse

Clonalité

Monoclonal

Isotype

IgG2a, kappa

ID de clone

SAA0522

Applications testées

FCM

Cible

Cold-insoluble globulin, CIG, FN, Fibronectin, FN1

Niveau d'endotoxines

< 10 EU/mg as determined by LAL method.

Pureté

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Numéro d'accès

P02751

RRID

Anti-Human FN1/Fibronectin Antibody (SAA0522) (abinScience Cat# HY259013, RRID:AB_3726039)

Forme

Liquid

Tampon de stockage

0.01M PBS pH 7.4

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Reconstitution

Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:2000-1:20000
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Contexte

Fibronectin (EDB) is a ~272 kDa protein. Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts. Acts as a ligand for the LILRB4 receptor, inhibiting FCGR1A/CD64-mediated monocyte activation.

1. Owens, RJ. et al. (1986) The EMBO journal 5, 2825-30. PMID: 3024962

2. Garcia-Pardo, A. et al. (1987) The Biochemical journal 241, 923-8. PMID: 3593230

3. Calaycay, J. et al. (1985) The Journal of biological chemistry 260, 12136-41. PMID: 3900070

4. Rostagno, A. et al. (1994) The Journal of biological chemistry 269, 31938-45. PMID: 7989369

5. Su, MT. et al. (2021) International immunology 33, 447-458. PMID: 34089617

Note

For research use only.

Isotype Control

Mouse IgG2a, kappa Recombinant Isotype Control Antibody (MOPC-173) (MF533507)

Images

SDS-PAGE

SDS-PAGE for Anti-Human FN1/Fibronectin Antibody (SAA0522)
Flow cytometry

Flow-cytometry using anti-human FN1 antibody. HepG2 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human FN1 monoclonal antibody (Catalog HY259013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.

Reférences

Fiche technique

Anti-Human Fibronectin Recombinant Antibody (SAA0522).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

Kits d'Essai

Anti-Human Fibronectin Recombinant Antibody (SAA0522) (HY259013)

Aperçu

Images

Reférences

Fiche technique

SDS