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| カタログ番号 | HY259013 | ||||||
|---|---|---|---|---|---|---|---|
| 説明 |
Anti-Human Fibronectin Recombinant Antibody (SAA0522) (HY259013) is a mouse monoclonal antibody detecting Fibronectin in ELISA, IF, IHC, SPR, WB, FCM. Suitable for Human, Mouse, Rat.
Highlights
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| 種反応性 | Human, Mouse, Rat | ||||||
| アプリケーション | ELISA, IF, IHC, SPR, WB, FCM | ||||||
| 宿主種 | Mouse | ||||||
| クローン性 | Monoclonal | ||||||
| アイソタイプ | IgG2a, kappa | ||||||
| クローンID | SAA0522 | ||||||
| テスト済みアプリケーション | FCM | ||||||
| ターゲット | Cold-insoluble globulin, CIG, FN, Fibronectin, FN1 | ||||||
| エンドトキシンレベル | < 10 EU/mg as determined by LAL method. | ||||||
| 純度 | >95% as determined by SDS-PAGE. | ||||||
| 精製 | Protein A/G purified from cell culture supernatant. | ||||||
| アクセッション番号 | P02751 | ||||||
| RRID | Anti-Human FN1/Fibronectin Antibody (SAA0522) (abinScience Cat# HY259013, RRID:AB_3726039) |
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| 形態 | Liquid | ||||||
| 保存バッファー | 0.01M PBS pH 7.4 データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 再構成 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. | ||||||
| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. | ||||||
| 背景 | Fibronectin (EDB) is a ~272 kDa protein. Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts. Acts as a ligand for the LILRB4 receptor, inhibiting FCGR1A/CD64-mediated monocyte activation. 1. Owens, RJ. et al. (1986) The EMBO journal 5, 2825-30. PMID: 3024962 2. Garcia-Pardo, A. et al. (1987) The Biochemical journal 241, 923-8. PMID: 3593230 3. Calaycay, J. et al. (1985) The Journal of biological chemistry 260, 12136-41. PMID: 3900070 4. Rostagno, A. et al. (1994) The Journal of biological chemistry 269, 31938-45. PMID: 7989369 5. Su, MT. et al. (2021) International immunology 33, 447-458. PMID: 34089617 | ||||||
| 注意事項 | For research use only. | ||||||
| Isotype Control | Mouse IgG2a, kappa Recombinant Isotype Control Antibody (MOPC-173) (MF533507) |
SDS-PAGE for Anti-Human FN1/Fibronectin Antibody (SAA0522)
Flow-cytometry using anti-human FN1 antibody. HepG2 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human FN1 monoclonal antibody (Catalog HY259013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.
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