Anti-Human Fibronectin Recombinant Antibody (SAA0522) (HY259013) is a mouse monoclonal antibody detecting Fibronectin in ELISA, IF, IHC, SPR, WB, FCM. Suitable for Human, Mouse, Rat.
Highlights
●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.
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Реконституция
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Информация об использовании продукта
Применение
Разбавление
ELISA
1:2000-1:20000
WB
1:500-1:2000
Устойчивость и хранение
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Фон
Fibronectin (EDB) is a ~272 kDa protein. Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts. Acts as a ligand for the LILRB4 receptor, inhibiting FCGR1A/CD64-mediated monocyte activation.
1. Owens, RJ. et al. (1986) The EMBO journal 5, 2825-30. PMID: 3024962
2. Garcia-Pardo, A. et al. (1987) The Biochemical journal 241, 923-8. PMID: 3593230
3. Calaycay, J. et al. (1985) The Journal of biological chemistry 260, 12136-41. PMID: 3900070
4. Rostagno, A. et al. (1994) The Journal of biological chemistry 269, 31938-45. PMID: 7989369
5. Su, MT. et al. (2021) International immunology 33, 447-458. PMID: 34089617
SDS-PAGE for Anti-Human FN1/Fibronectin Antibody (SAA0522)
Flow cytometry
Flow-cytometry using anti-human FN1 antibody. HepG2 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human FN1 monoclonal antibody (Catalog HY259013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.
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