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Anti-Human HCRTR1 Polyclonal Antibody (HT363014)

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Vista general
Número de catálogoHT363014
Descripción
Anti-Human HCRTR1 Polyclonal Antibody (HT363014) is a rabbit polyclonal antibody detecting HCRTR1/OX1R in ELISA, IHC, WB. Suitable for Human, Mouse, Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactividad de especiesHuman, Mouse, Rat
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human HCRTR1 (Gln179-Lys213).
Objetivo Hypocretin receptor type 1, Ox1-R, Ox1R, Ox-1-R, Orexin receptor type 1, HCRTR1
Purificación Purified by antigen affinity column.
Número de acceso O43613
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Orexin/Hypocretin receptor type 1 (HCRTR1/OX1R) is a ~47 kDa protein. G-protein coupled receptor that binds the neuropeptide orexin-A with high affinity, and orexin-B with lower affinity, two peptides derived from a common precursor, prepro-orexin. Its activity is mediated via a G(q)-protein-coupled pathway, which activates the phosphatidylinositol-calcium second messenger system in response to orexin-A binding. In addition to G(q)-mediated signaling, orexin-A stimulation also promotes beta-arrestin recruitment, leading to receptor internalization. Plays a significant role in the regulation of food intake.

1. Hellmann, J. et al. (2020) Proceedings of the National Academy of Sciences of the United States of America 117, 18059-18067. PMID: 32669442
2. Sakurai, T. et al. (1998) Cell 92, 573-85. PMID: 9491897
3. Milasta, S. et al. (2005) The Biochemical journal 387, 573-84. PMID: 15683363
Nota For research use only.
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Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human HCRTR1 Polyclonal Antibody

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