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Anti-Human HGF Recombinant Antibody (SAA0434) (HB191107)

Anti-Human HGF Recombinant Antibody (SAA0434)
Anti-Human HGF Recombinant Antibody (SAA0434)
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Vista general
Número de catálogoHB191107
Reactividad de especiesHuman
AplicacionesELISA, FCM
Especie huéspedMouse
ClonalidadMonoclonal
IsotipoIgG2a, kappa
ID de clonSAA0434
Aplicaciones probadas FCM
Objetivo SF, HPTA, Hepatopoietin-A, Hepatocyte growth factor, Scatter factor, HGF, HGFK1, NK1 domain of HGF
Nivel de endotoxinas Please contact with the lab for this information.
Pureza >95% as determined by SDS-PAGE.
Purificación Protein A/G purified from cell culture supernatant.
Número de acceso P14210
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 0.09% Sodium azide.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Fondo

Hepatocyte growth factor (HGF) is a ~83 kDa protein. Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization. Activates MAPK signaling following TMPRSS13 cleavage and activation.

1. Tolbert, WD. et al. (2010) Proceedings of the National Academy of Sciences of the United States of America 107, 13264-9. PMID: 20624990
2. Stamos, J. et al. (2004) The EMBO journal 23, 2325-35. PMID: 15167892
3. Hashimoto, T. et al. (2010) The FEBS journal 277, 4888-900. PMID: 20977675
Nota For research use only.
Imágenes
  • Anti-Human HGF Recombinant Antibody (SAA0434)

    Flow Cytometry

    Flow-cytometry using anti-human HGF antibody.U87 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human HGF antibody monoclonal antibody (Catalog # HB191107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

Referencias
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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