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Anti-Human HGF Recombinant Antibody (SAA0434) (HB191107)

Anti-Human HGF Recombinant Antibody (SAA0434)
Anti-Human HGF Recombinant Antibody (SAA0434)
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概要
カタログ番号HB191107
種反応性Human
アプリケーションELISA, FCM
宿主種Mouse
クローン性Monoclonal
アイソタイプIgG2a, kappa
クローンIDSAA0434
テスト済みアプリケーション FCM
ターゲット SF, HPTA, Hepatopoietin-A, Hepatocyte growth factor, Scatter factor, HGF, HGFK1, NK1 domain of HGF
エンドトキシンレベル Please contact with the lab for this information.
純度 >95% as determined by SDS-PAGE.
精製 Protein A/G purified from cell culture supernatant.
アクセッション番号 P14210
形態 Liquid
保存バッファー 0.01M PBS, pH 7.4, 0.09% Sodium azide.

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

安定性と保存 Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
背景

Hepatocyte growth factor (HGF) is a ~83 kDa protein. Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization. Activates MAPK signaling following TMPRSS13 cleavage and activation.

1. Tolbert, WD. et al. (2010) Proceedings of the National Academy of Sciences of the United States of America 107, 13264-9. PMID: 20624990
2. Stamos, J. et al. (2004) The EMBO journal 23, 2325-35. PMID: 15167892
3. Hashimoto, T. et al. (2010) The FEBS journal 277, 4888-900. PMID: 20977675
注意事項 For research use only.
イメージ
  • Anti-Human HGF Recombinant Antibody (SAA0434)

    Flow Cytometry

    Flow-cytometry using anti-human HGF antibody.U87 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human HGF antibody monoclonal antibody (Catalog # HB191107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

参考文献
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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C₁ (Stock Conc.)
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