Anti-Human KARS1/Lysyl-tRNA synthetase Recombinant Antibody (SAA1945) (HC295013)

Name: Anti-Human KARS1/Lysyl-tRNA synthetase Recombinant Antibody (SAA1945)
Brand: abinScience
SKU: HC295013
Price: 196 USD
Availability: InStock

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50ug
100ug
1mg

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Aperçu
Images
Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

HC295013

Reactivité des espèces

Human

Applications

ELISA

Espèce hôte

Mouse

Clonalité

Monoclonal

Isotype

IgG2a, kappa

ID de clone

SAA1945

Cible

KIAA0070, KARS, Lysyl-tRNA synthetase, LysRS, Lysine--tRNA ligase, KARS1

Niveau d'endotoxines

Please contact with the lab for this information.

Pureté

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Numéro d'accès

Q15046

RRID

Anti-Human KARS1/Lysyl-tRNA synthetase Antibody (SAA1945) (abinScience Cat# HC295013, RRID:AB_3727709)
_x005F_x005F_x000D_

Forme

Liquid

Tampon de stockage

0.01M PBS pH 7.4

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:2000-1:20000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Contexte

Lysine--tRNA ligase (KARS1) is a ~68 kDa protein. Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. When secreted, acts as a signaling molecule that induces immune response through the activation of monocyte/macrophages. Catalyzes the synthesis of the signaling molecule diadenosine tetraphosphate (Ap4A), and thereby mediates disruption of the complex between HINT1 and MITF and the concomitant activation of MITF transcriptional activity.

1. Guzzo, CM. et al. (2008) Biochemical and biophysical research communications 365, 718-23. PMID: 18029264

2. Guo, M. et al. (2008) Proceedings of the National Academy of Sciences of the United States of America 105, 2331-6. PMID: 18272479

3. Shiba, K. et al. (1997) The Journal of biological chemistry 272, 22809-16. PMID: 9278442

4. Park, SG. et al. (2005) Proceedings of the National Academy of Sciences of the United States of America 102, 6356-61. PMID: 15851690

5. Lee, YN. et al. (2004) Immunity 20, 145-51. PMID: 14975237

6. Yannay-Cohen, N. et al. (2009) Molecular cell 34, 603-11. PMID: 19524539

7. Ofir-Birin, Y. et al. (2013) Molecular cell 49, 30-42. PMID: 23159739

8. Zamecnik, PC. et al. (1966) Biochemical and biophysical research communications 24, 91-7. PMID: 5338216

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Human KARS1/Lysyl-tRNA synthetase Antibody (SAA1945)

Reférences

Fiche technique

Anti-Human KARS1/Lysyl-tRNA synthetase Recombinant Antibody (SAA1945).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

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