Anti-KARS1 Polyclonal Antibody (HC295014)

Name: Anti-KARS1 Polyclonal Antibody
Brand: abinScience
SKU: HC295014
Price: 168 USD
Availability: InStock

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Overview

Catalog No.

HC295014

Description

Anti-KARS1 Polyclonal Antibody (HC295014) is a rabbit polyclonal antibody detecting KARS1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, and Cricetulus griseus.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human KARS1 (Arg323-Arg553).

Target

KIAA0070, KARS, Lysyl-tRNA synthetase, LysRS, Lysine--tRNA ligase, KARS1

Purification

Purified by antigen affinity column.

Accession

Q15046

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Lysine--tRNA ligase (KARS1) is a ~68 kDa protein. Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. When secreted, acts as a signaling molecule that induces immune response through the activation of monocyte/macrophages. Catalyzes the synthesis of the signaling molecule diadenosine tetraphosphate (Ap4A), and thereby mediates disruption of the complex between HINT1 and MITF and the concomitant activation of MITF transcriptional activity.

1. Guzzo, CM. et al. (2008) Biochemical and biophysical research communications 365, 718-23. PMID: 18029264

2. Guo, M. et al. (2008) Proceedings of the National Academy of Sciences of the United States of America 105, 2331-6. PMID: 18272479

3. Shiba, K. et al. (1997) The Journal of biological chemistry 272, 22809-16. PMID: 9278442

4. Park, SG. et al. (2005) Proceedings of the National Academy of Sciences of the United States of America 102, 6356-61. PMID: 15851690

5. Lee, YN. et al. (2004) Immunity 20, 145-51. PMID: 14975237

6. Yannay-Cohen, N. et al. (2009) Molecular cell 34, 603-11. PMID: 19524539

7. Ofir-Birin, Y. et al. (2013) Molecular cell 49, 30-42. PMID: 23159739

8. Zamecnik, PC. et al. (1966) Biochemical and biophysical research communications 24, 91-7. PMID: 5338216

Note

For research use only.

Images

References

Datasheet

Anti-KARS1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Assay Kits

Anti-KARS1 Polyclonal Antibody (HC295014)

Overview

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References

Datasheet

SDS

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