Anti-Human KARS1/Lysyl-tRNA synthetase Recombinant Antibody (SAA1945) (HC295013)

Name: Anti-Human KARS1/Lysyl-tRNA synthetase Recombinant Antibody (SAA1945)
Brand: abinScience
SKU: HC295013
Price: 196 USD
Availability: InStock

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50ug
100ug
1mg

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HC295013

Species reactivity

Human

Applications

ELISA

Host species

Mouse

Isotype

IgG2a, kappa

Clone ID

SAA1945

Clonality

Monoclonal

Target

KIAA0070, KARS, Lysyl-tRNA synthetase, LysRS, Lysine--tRNA ligase, KARS1

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q15046

RRID

Anti-Human KARS1/Lysyl-tRNA synthetase Antibody (SAA1945) (abinScience Cat# HC295013, RRID:AB_3727709)
_x005F_x000D_

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Lysine--tRNA ligase (KARS1) is a ~68 kDa protein. Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. When secreted, acts as a signaling molecule that induces immune response through the activation of monocyte/macrophages. Catalyzes the synthesis of the signaling molecule diadenosine tetraphosphate (Ap4A), and thereby mediates disruption of the complex between HINT1 and MITF and the concomitant activation of MITF transcriptional activity.

1. Guzzo, CM. et al. (2008) Biochemical and biophysical research communications 365, 718-23. PMID: 18029264

2. Guo, M. et al. (2008) Proceedings of the National Academy of Sciences of the United States of America 105, 2331-6. PMID: 18272479

3. Shiba, K. et al. (1997) The Journal of biological chemistry 272, 22809-16. PMID: 9278442

4. Park, SG. et al. (2005) Proceedings of the National Academy of Sciences of the United States of America 102, 6356-61. PMID: 15851690

5. Lee, YN. et al. (2004) Immunity 20, 145-51. PMID: 14975237

6. Yannay-Cohen, N. et al. (2009) Molecular cell 34, 603-11. PMID: 19524539

7. Ofir-Birin, Y. et al. (2013) Molecular cell 49, 30-42. PMID: 23159739

8. Zamecnik, PC. et al. (1966) Biochemical and biophysical research communications 24, 91-7. PMID: 5338216

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Human KARS1/Lysyl-tRNA synthetase Antibody (SAA1945)

References

Datasheet

Anti-Human KARS1/Lysyl-tRNA synthetase Recombinant Antibody (SAA1945).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human KARS1/Lysyl-tRNA synthetase Recombinant Antibody (SAA1945) (HC295013)

Overview

Images

References

Datasheet

SDS

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