Anti-Human PCNA Polyclonal Antibody (HY337014)

Name: Anti-Human PCNA Polyclonal Antibody
Brand: abinScience
SKU: HY337014
Price: 96 USD
Availability: InStock

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Vista general

Número de catálogo

HY337014

Descripción

Anti-Human PCNA Polyclonal Antibody (HY337014) is a rabbit polyclonal antibody detecting PCNA in ELISA, IHC, WB. Suitable for Rattus norvegicus, Coturnix japonica, Xenopus laevis, Anguilla japonica, and Bovine.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactividad de especies

Human, Mouse, Rat

Aplicaciones

ELISA, IHC, WB

Especie huésped

Rabbit

Clonalidad

Polyclonal

Isotipo

IgG

Inmunógeno

E. coli - derived recombinant Human PCNA (Met1-Ser261).

Objetivo

PCNA

Purificación

Purified by antigen affinity column.

Número de acceso

P12004

Forma

Liquid

Buffer de almacenamiento

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Nombres alternativos

Proliferating cell nuclear antigen, PCNA, Cyclin, PCNA

Fondo

DNA sliding clamp PCNA is a ~28 kDa protein. Confers DNA tethering and processivity to DNA polymerases and other proteins. Auxiliary protein of DNA polymerase delta and epsilon, is involved in the control of DNA replication by increasing the polymerases' processivity during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways.

1. Nicolae, CM. et al. (2014) The Journal of biological chemistry 289, 13627-37. PMID: 24695737

2. Cazzalini, O. et al. (2014) Nucleic acids research 42, 8433-48. PMID: 24939902

3. Baris, Y. et al. (2022) Nature 606, 204-210. PMID: 35585232

Nota

For research use only.

Imágenes

Western blot

Western blot analysis was performed using anti-PCNA polyclonal antibody at 1ug/mL on various samples.
Lane 1: NIH3T3 cell lysate
Lane 2: PC-12 cell lysate
Lane 3: Hela cell lysate
Lane 4: MCF-7 cell lysate
Lane 5: HepG2 cell lysate
Lane 6: Jurkat cell lysate
Lane 7: 293T cell lysate
Lane 8: Mouse spleen lysate