Anti-PCNA Recombinant Antibody (SAA2241) (HY337013)

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Price(USD): $

Spec:

100ug
1mg

Number:

Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HY337013

Description

Anti-PCNA Antibody (SAA2241) [SAA2241] (HY337013) is a human monoclonal antibody detecting PCNA in ELISA, WB. Suitable for Human.

Species reactivity

Human

Applications

ELISA, WB

Host species

Human

Isotype

IgG1, kappa

Clone ID

SAA2241

Clonality

Monoclonal

Target

PCNA

Endotoxin level

Please contact with the lab for this information.

Purity

>90% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P12004

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Alternate Names

Cyclin, Proliferating cell nuclear antigen, PCNA

Background

DNA sliding clamp PCNA is a ~28 kDa protein. Confers DNA tethering and processivity to DNA polymerases and other proteins. Auxiliary protein of DNA polymerase delta and epsilon, is involved in the control of DNA replication by increasing the polymerases' processivity during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways.

1. Nicolae, CM. et al. (2014) The Journal of biological chemistry 289, 13627-37. PMID: 24695737

2. Cazzalini, O. et al. (2014) Nucleic acids research 42, 8433-48. PMID: 24939902

3. Baris, Y. et al. (2022) Nature 606, 204-210. PMID: 35585232

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-PCNA monoclonal antibody at 1ug/mL on various samples.
Lane 1: NIH3T3 cell lysate
Lane 2: PC-12 cell lysate
Lane 3: Hela cell lysate
Lane 4: MCF-7 cell lysate
Lane 5: HepG2 cell lysate
Lane 6: Jurkat cell lysate
Lane 7: 293T cell lysate
Lane 8: Mouse spleen lysate
Lane 9: Rat spleen lysate
Western blot

Western blot analysis was performed using anti-PCNA monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human PCNA (Catalog No: HY337012)
Lane 2: negative control
Bioactivity

Detects recombinant human PCNA (Catalog No: HY337012) in indirect ELISA