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| カタログ番号 | HY337014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 説明 |
Anti-Human PCNA Polyclonal Antibody (HY337014) is a rabbit polyclonal antibody detecting PCNA in ELISA, IHC, WB. Suitable for Rattus norvegicus, Coturnix japonica, Xenopus laevis, Anguilla japonica, and Bovine.
Highlights
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| 種反応性 | Human, Mouse, Rat | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Human PCNA (Met1-Ser261). | ||||||||
| ターゲット | PCNA | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | P12004 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 別名 | Proliferating cell nuclear antigen, PCNA, Cyclin, PCNA | ||||||||
| 背景 | DNA sliding clamp PCNA is a ~28 kDa protein. Confers DNA tethering and processivity to DNA polymerases and other proteins. Auxiliary protein of DNA polymerase delta and epsilon, is involved in the control of DNA replication by increasing the polymerases' processivity during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. 1. Nicolae, CM. et al. (2014) The Journal of biological chemistry 289, 13627-37. PMID: 24695737 2. Cazzalini, O. et al. (2014) Nucleic acids research 42, 8433-48. PMID: 24939902 3. Baris, Y. et al. (2022) Nature 606, 204-210. PMID: 35585232 | ||||||||
| 注意事項 | For research use only. |
Western blot analysis was performed using anti-PCNA polyclonal antibody at 1ug/mL on various samples.
Lane 1: NIH3T3 cell lysate
Lane 2: PC-12 cell lysate
Lane 3: Hela cell lysate
Lane 4: MCF-7 cell lysate
Lane 5: HepG2 cell lysate
Lane 6: Jurkat cell lysate
Lane 7: 293T cell lysate
Lane 8: Mouse spleen lysate
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