Anti-Human Podocalyxin Like Recombinant Antibody (Mab47) (HT033107)

Name: Anti-Human Podocalyxin Like Recombinant Antibody (Mab47)
Brand: abinScience
SKU: HT033107
Price: 191 USD
Availability: InStock

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Vista general

Número de catálogo	HT033107
Reactividad de especies	Human
Aplicaciones	ELISA, FCM, IHC, Neutralization
Especie huésped	Mouse
Clonalidad	Monoclonal
Isotipo	IgG2a, kappa
ID de clon	Mab47
Objetivo	PCLP1, Podocalyxin-like protein 1, GCTM-2 antigen, Gp200, PODXL, Podocalyxin, PC, PCLP, PCLP-1
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>95% as determined by SDS-PAGE.
Purificación	Protein A/G purified from cell culture supernatant.
Número de acceso	O00592
Forma	Liquid
Buffer de almacenamiento	0.01M PBS pH 7.4 Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Fondo	Podocalyxin (PODXL) is a ~58 kDa protein. Involved in the regulation of both adhesion and cell morphology and cancer progression. Functions as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell-cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up initial epithelial polarization and the apical lumen formation during renal tubulogenesis.
Nota	For research use only.

Imágenes

Bioactivity

Detects recombinant human PODXL (Cat No.: HT033012) in indirect ELISA.
Western blot

Western blot analysis was performed using anti-PODXL monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human PODXL (Catalog No: HT033012)
Lane 2: negative control
Flow cytometry

Flow-cytometry using anti-human PODXL antibody. Hela cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human PODXL monoclonal antibody (Catalog HT033107, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.