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Anti-Human PODXL Antibody (Mab47), FITC (HT033117)

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Overview
Catalog No.HT033117
Description
Anti-Human PODXL Antibody (Mab47), FITC [Mab47] (HT033117) is a mouse monoclonal antibody detecting PODXL in ELISA, FCM. Suitable for Human.
Highlights
  • Flow Cytometry — Validated for flow cytometry applications.
  • Fluorescent Label — Direct conjugate for streamlined workflows.
Species reactivityHuman
ApplicationsELISA, FCM
Host speciesMouse
IsotypeIgG2a, kappa
Clone IDMab47
ConjugationFITC
Clonality Monoclonal
Target PCLP1, Podocalyxin-like protein 1, GCTM-2 antigen, Gp200, PODXL, Podocalyxin, PC, PCLP, PCLP-1
Endotoxin level Please contact with the lab for this information.
Accession O00592
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Stability and Storage Store at 4°C for 12 months. Protect from light. Do not freeze.
Background

Podocalyxin (PODXL) is a ~58 kDa protein. Involved in the regulation of both adhesion and cell morphology and cancer progression. Functions as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell-cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up initial epithelial polarization and the apical lumen formation during renal tubulogenesis.

Note For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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