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Recombinant Human PODXL Protein, N-His (HT033012)

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Recombinant Human PODXL Protein, N-His

Recombinant Human PODXL Protein, N-His

Recombinant Human PODXL Protein, N-His

Recombinant Human PODXL Protein, N-His

Recombinant Human PODXL Protein, N-His

Recombinant Human PODXL Protein, N-His

Recombinant Human PODXL Protein, N-His

Recombinant Human PODXL Protein, N-His

Recombinant Human PODXL Protein, N-His

Recombinant Human PODXL Protein, N-His

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100ug
1mg

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Overview
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References
Datasheet

Overview

Catalog No.	HT033012
Description	Recombinant Human PODXL Protein, N-His (HT033012) expressed in E. coli, spanning Ser23-Leu428. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	O00592
Protein length	Ser23-Leu428
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	PCLP1, Podocalyxin-like protein 1, GCTM-2 antigen, Gp200, PODXL, Podocalyxin, PC, PCLP, PCLP-1
Background	Podocalyxin (PODXL) is a ~58 kDa protein. Involved in the regulation of both adhesion and cell morphology and cancer progression. Functions as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell-cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up initial epithelial polarization and the apical lumen formation during renal tubulogenesis.
Note	For research use only.

Images

SDS-PAGE

SDS-PAGE for Recombinant Human PODXL Protein, N-His
Bioactivity

Detected by Anti-Human PODXL Antibody (Mab47) (Cat No.: HT033107) inindirectELISA.
Western blot

Detected by Anti-PODXL monoclonal antibody (Catalog No: HT033207)
Western blot

Detected by Anti-PODXL monoclonal antibody (Catalog No: HT033107)
Western blot

Detected by Anti-PODXL monoclonal antibody (Catalog No: HT033016)

References

HT033012-SDS-PAGE-1.jpg

Datasheet

Recombinant Human PODXL Protein, N-His.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Stock Solution

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Recombinant Human PODXL Protein, N-His