Anti-Human Podocalyxin Like Recombinant Antibody (Mab60) (HT033207)

Name: Anti-Human Podocalyxin Like Recombinant Antibody (Mab60)
Brand: abinScience
SKU: HT033207
Price: 191 USD
Availability: InStock

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概要
イメージ
参考文献
データシート
SDS

概要

カタログ番号	HT033207
種反応性	Human
アプリケーション	ELISA, FCM, IHC, Neutralization
宿主種	Mouse
クローン性	Monoclonal
アイソタイプ	IgG2a, kappa
クローンID	Mab60
ターゲット	PCLP1, Podocalyxin-like protein 1, GCTM-2 antigen, Gp200, PODXL, Podocalyxin, PC, PCLP, PCLP-1
エンドトキシンレベル	Please contact with the lab for this information.
純度	>95% as determined by SDS-PAGE.
精製	Protein A/G purified from cell culture supernatant.
アクセッション番号	O00592
形態	Liquid
保存バッファー	0.01M PBS pH 7.4 データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。
安定性と保存	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
背景	Podocalyxin (PODXL) is a ~58 kDa protein. Involved in the regulation of both adhesion and cell morphology and cancer progression. Functions as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell-cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up initial epithelial polarization and the apical lumen formation during renal tubulogenesis.
注意事項	For research use only.

イメージ

Western blot

Western blot analysis was performed using anti-PODXL monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human PODXL (Catalog No: HT033012)
Lane 2: negative control
Flow cytometry

Flow-cytometry using anti-human PODXL antibody. Hela cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human PODXL monoclonal antibody (Catalog HT033207, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.