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| カタログ番号 | HP485023 | ||||
|---|---|---|---|---|---|
| 種反応性 | Human | ||||
| アプリケーション | ELISA | ||||
| 宿主種 | Human | ||||
| クローン性 | Monoclonal | ||||
| アイソタイプ | IgG1, kappa | ||||
| クローンID | B5209B | ||||
| ターゲット | ROBO1, Roundabout homolog 1, H-Robo-1 | ||||
| エンドトキシンレベル | Please contact with the lab for this information. | ||||
| 純度 | >95% as determined by SDS-PAGE. | ||||
| 精製 | Protein A/G purified from cell culture supernatant. | ||||
| アクセッション番号 | Q9Y6N7 | ||||
| RRID | Anti-Human ROBO1 Antibody (B5209B) (abinScience Cat# HP485023, RRID:AB_3727777) |
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| 形態 | Liquid | ||||
| 保存バッファー | 0.01M PBS pH 7.4 データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. | ||||
| 背景 | Roundabout homolog 1 (ROBO1) is a ~180 kDa protein. Receptor for SLIT1 and SLIT2 that mediates cellular responses to molecular guidance cues in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development. Interaction with the intracellular domain of FLRT3 mediates axon attraction towards cells expressing NTN1. In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex. Plays a role in the regulation of cell migration via its interaction with MYO9B; inhibits MYO9B-mediated stimulation of RHOA GTPase activity, and thereby leads to increased levels of active, GTP-bound RHOA. May be required for lung development. 1. Brose, K. et al. (1999) Cell 96, 795-806. PMID: 10102268 2. Leyva-Díaz, E. et al. (2014) Current biology : CB 24, 494-508. PMID: 24560577 3. Kong, R. et al. (2015) The Journal of clinical investigation 125, 4407-20. PMID: 26529257 | ||||
| 注意事項 | For research use only. | ||||
| Isotype Control | Human IgG1 (LALAPG) Recombinant Isotype Control Antibody (HyHEL-10) (HP688307) |
Flow-cytometry using FITC anti-human ROBO1 antibody. HepG-2 cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human ROBO1 monoclonal antibody (Catalog: HP485023, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Western blot analysis was performed using anti-ROBO1 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human ROBO1 (Catalog No: HP485011)
Lane 2: negative control
Detects recombinant human ROBO1 (Catalog No: HP485011) in indirect ELISA
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