Human ROBO1 Recombinant Protein (C-His) (HP485011)

Name: Human ROBO1 Recombinant Protein (C-His)
Brand: abinScience
SKU: HP485011
Price: 298 USD
Availability: InStock

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Overview
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Datasheet
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Overview

Catalog No.	HP485011
Description	Recombinant Human ROBO1 Protein, C-His (HP485011) expressed in Mammalian Cells, spanning Gln26-Pro897. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●Mammalian Expression — Proper folding and native glycosylation. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	Mammalian cells
Accession	Q9Y6N7
Protein length	Gln26-Pro897
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	98.52 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Roundabout homolog 1, Deleted in U twenty twenty, H-Robo-1, ROBO1, DUTT1
Background	Roundabout homolog 1 is a ~180 kDa protein. Receptor for SLIT1 and SLIT2 that mediates cellular responses to molecular guidance cues in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development. Interaction with the intracellular domain of FLRT3 mediates axon attraction towards cells expressing NTN1. In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex. Plays a role in the regulation of cell migration via its interaction with MYO9B; inhibits MYO9B-mediated stimulation of RHOA GTPase activity, and thereby leads to increased levels of active, GTP-bound RHOA. May be required for lung development. 1. Brose, K. et al. (1999) Cell 96, 795-806. PMID: 10102268 2. Leyva-Díaz, E. et al. (2014) Current biology : CB 24, 494-508. PMID: 24560577 3. Kong, R. et al. (2015) The Journal of clinical investigation 125, 4407-20. PMID: 26529257
Note	For research use only

Images

Western blot

Detected by Anti-ROBO1 monoclonal antibody (Catalog No: HP485023)
Western blot

Detected by Anti-ROBO1 monoclonal antibody (Catalog No: HP485013)
Bioactivity

Detected by Anti-Human ROBO1 Antibody (B5209B) (Catalog No: HP485023) in indirect ELISA
Bioactivity

Detected by SAA1494 (Catalog No: HP485013) in indirect ELISA.