





| Catalog No. | HP485023 | ||||
|---|---|---|---|---|---|
| Species reactivity | Human | ||||
| Applications | ELISA | ||||
| Host species | Human | ||||
| Isotype | IgG1, kappa | ||||
| Clone ID | B5209B | ||||
| Clonality | Monoclonal | ||||
| Target | ROBO1, Roundabout homolog 1, H-Robo-1 | ||||
| Endotoxin level | Please contact with the lab for this information. | ||||
| Purity | >95% as determined by SDS-PAGE. | ||||
| Purification | Protein A/G purified from cell culture supernatant. | ||||
| Accession | Q9Y6N7 | ||||
| Form | Liquid | ||||
| Storage buffer | 0.01M PBS, pH 7.4. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
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| Product Usage Information |
| ||||
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. | ||||
| Note | For research use only. |

Flow-cytometry using FITC anti-human ROBO1 antibody. HepG-2 cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human ROBO1 monoclonal antibody (Catalog: HP485023, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

Western blot analysis was performed using anti-ROBO1 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human ROBO1 (Catalog No: HP485011)
Lane 2: negative control

Detects recombinant human ROBO1 (Catalog No: HP485011) in indirect ELISA




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