Anti-Human ROBO1 Recombinant Antibody (B5209B) (HP485023)

Name: Anti-Human ROBO1 Recombinant Antibody (B5209B)
Brand: abinScience
SKU: HP485023
Price: 110 USD
Availability: InStock

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1mg

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HP485023

Species reactivity

Human

Applications

ELISA

Host species

Human

Isotype

IgG1, kappa

Clone ID

B5209B

Clonality

Monoclonal

Target

ROBO1, Roundabout homolog 1, H-Robo-1

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q9Y6N7

RRID

Anti-Human ROBO1 Antibody (B5209B) (abinScience Cat# HP485023, RRID:AB_3727777)
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Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Roundabout homolog 1 (ROBO1) is a ~180 kDa protein. Receptor for SLIT1 and SLIT2 that mediates cellular responses to molecular guidance cues in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development. Interaction with the intracellular domain of FLRT3 mediates axon attraction towards cells expressing NTN1. In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex. Plays a role in the regulation of cell migration via its interaction with MYO9B; inhibits MYO9B-mediated stimulation of RHOA GTPase activity, and thereby leads to increased levels of active, GTP-bound RHOA. May be required for lung development.

1. Brose, K. et al. (1999) Cell 96, 795-806. PMID: 10102268

2. Leyva-Díaz, E. et al. (2014) Current biology : CB 24, 494-508. PMID: 24560577

3. Kong, R. et al. (2015) The Journal of clinical investigation 125, 4407-20. PMID: 26529257

Note

For research use only.

Images

Flow CytoMetry

Flow-cytometry using FITC anti-human ROBO1 antibody. HepG-2 cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human ROBO1 monoclonal antibody (Catalog: HP485023, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Western blot

Western blot analysis was performed using anti-ROBO1 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human ROBO1 (Catalog No: HP485011)
Lane 2: negative control
Bioactivity

Detects recombinant human ROBO1 (Catalog No: HP485011) in indirect ELISA