Anti-Human ROBO1 Recombinant Antibody (B5209B) (HP485023)

Name: Anti-Human ROBO1 Recombinant Antibody (B5209B)
Brand: abinScience
SKU: HP485023
Price: 110 USD
Availability: InStock

Даташит Поделиться продуктом Копировать информацию

Цена(USD):

Спецификация:

20ug
50ug
100ug
1mg

Количество:

Свяжитесь с нами

Обзор
Изображения
Ссылки
Даташит
SDS

Обзор

Каталожный номер

HP485023

Реактивность видов

Human

Применения

ELISA

Хозяинский вид

Human

Клоональность

Monoclonal

Изотип

IgG1, kappa

ID клона

B5209B

Цель

ROBO1, Roundabout homolog 1, H-Robo-1

Уровень эндотоксинов

Please contact with the lab for this information.

Чистота

>95% as determined by SDS-PAGE.

Очистка

Protein A/G purified from cell culture supernatant.

Номер доступа

Q9Y6N7

RRID

Anti-Human ROBO1 Antibody (B5209B) (abinScience Cat# HP485023, RRID:AB_3727777)
_x005F_x005F_x000D_

Форма

Liquid

Буфер хранения

0.01M PBS pH 7.4

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта

Применение	Разбавление
ELISA	1:2000-1:20000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Фон

Roundabout homolog 1 (ROBO1) is a ~180 kDa protein. Receptor for SLIT1 and SLIT2 that mediates cellular responses to molecular guidance cues in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development. Interaction with the intracellular domain of FLRT3 mediates axon attraction towards cells expressing NTN1. In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex. Plays a role in the regulation of cell migration via its interaction with MYO9B; inhibits MYO9B-mediated stimulation of RHOA GTPase activity, and thereby leads to increased levels of active, GTP-bound RHOA. May be required for lung development.

1. Brose, K. et al. (1999) Cell 96, 795-806. PMID: 10102268

2. Leyva-Díaz, E. et al. (2014) Current biology : CB 24, 494-508. PMID: 24560577

3. Kong, R. et al. (2015) The Journal of clinical investigation 125, 4407-20. PMID: 26529257

Примечание

For research use only.

Isotype Control

Human IgG1 (LALAPG) Recombinant Isotype Control Antibody (HyHEL-10) (HP688307)

Изображения

Flow CytoMetry

Flow-cytometry using FITC anti-human ROBO1 antibody. HepG-2 cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human ROBO1 monoclonal antibody (Catalog: HP485023, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Western blot

Western blot analysis was performed using anti-ROBO1 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human ROBO1 (Catalog No: HP485011)
Lane 2: negative control
Bioactivity

Detects recombinant human ROBO1 (Catalog No: HP485011) in indirect ELISA