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Anti-Human TLR5 Polyclonal Antibody (HT266014)

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Vista general
Número de catálogoHT266014
Descripción
Anti-Human TLR5 Polyclonal Antibody (HT266014) is a rabbit polyclonal antibody detecting CD285/TLR5 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactividad de especiesHuman
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human TLR5 (Ile21-Phe115).
Objetivo TIL3, TLR5, Toll/interleukin-1 receptor-like protein 3, Toll-like receptor 5
Purificación Purified by antigen affinity column.
Número de acceso O60602
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Toll-like receptor 5 (CD285/TLR5) is a ~97 kDa protein. Pattern recognition receptor (PRR) located on the cell surface that participates in the activation of innate immunity and inflammatory response. Recognizes small molecular motifs named pathogen-associated molecular pattern (PAMPs) expressed by pathogens and microbe-associated molecular patterns (MAMPs) usually expressed by resident microbiota. Upon ligand binding such as bacterial flagellins, recruits intracellular adapter proteins MYD88 and TRIF leading to NF-kappa-B activation, cytokine secretion and induction of the inflammatory response. Plays thereby an important role in the relationship between the intestinal epithelium and enteric microbes and contributes to the gut microbiota composition throughout life.

1. Hayashi, F. et al. (2001) Nature 410, 1099-103. PMID: 11323673
2. Blohmke, CJ. et al. (2008) Journal of immunology (Baltimore, Md. : 1950) 180, 7764-73. PMID: 18490781
3. Bielaszewska, M. et al. (2018) International journal of medical microbiology : IJMM 308, 882-889. PMID: 29934223
4. Gewirtz, AT. et al. (2001) Journal of immunology (Baltimore, Md. : 1950) 167, 1882-5. PMID: 11489966
5. Choi, YJ. et al. (2010) The Journal of biological chemistry 285, 37570-8. PMID: 20855887
Nota For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human TLR5 Polyclonal Antibody

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