Anti-Human CD285/TLR5 Polyclonal Antibody (HT266024)

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50ug
100ug

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Overview

Catalog No.

HT266024

Description

Anti-Human CD285/TLR5 Polyclonal Antibody (HT266024) is a rabbit polyclonal antibody detecting TLR5 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CD285/TLR5 (Thr661-Ser858).

Target

TIL3, TLR5, Toll/interleukin-1 receptor-like protein 3, Toll-like receptor 5

Purification

Purified by antigen affinity column.

Accession

O60602

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Toll-like receptor 5 (CD285/TLR5) is a ~97 kDa protein. Pattern recognition receptor (PRR) located on the cell surface that participates in the activation of innate immunity and inflammatory response. Recognizes small molecular motifs named pathogen-associated molecular pattern (PAMPs) expressed by pathogens and microbe-associated molecular patterns (MAMPs) usually expressed by resident microbiota. Upon ligand binding such as bacterial flagellins, recruits intracellular adapter proteins MYD88 and TRIF leading to NF-kappa-B activation, cytokine secretion and induction of the inflammatory response. Plays thereby an important role in the relationship between the intestinal epithelium and enteric microbes and contributes to the gut microbiota composition throughout life.

1. Hayashi, F. et al. (2001) Nature 410, 1099-103. PMID: 11323673

2. Blohmke, CJ. et al. (2008) Journal of immunology (Baltimore, Md. : 1950) 180, 7764-73. PMID: 18490781

3. Bielaszewska, M. et al. (2018) International journal of medical microbiology : IJMM 308, 882-889. PMID: 29934223

4. Gewirtz, AT. et al. (2001) Journal of immunology (Baltimore, Md. : 1950) 167, 1882-5. PMID: 11489966

5. Choi, YJ. et al. (2010) The Journal of biological chemistry 285, 37570-8. PMID: 20855887

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-Human CD285/TLR5 polyclonal antibody at 1ug/mL on recombinant Human CD285/TLR5 (Catalog No. HT266022).

References

Datasheet