Human CD285/TLR5 Recombinant Protein (N-GST) (HT266022)

Name: Human CD285/TLR5 Recombinant Protein (N-GST)
Brand: abinScience
SKU: HT266022
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HT266022
Description	Recombinant Human CD285/TLR5 Protein, N-GST (HT266022) expressed in E. coli, spanning Thr661-Ser858. Purity: >90% by SDS-PAGE. Highlights ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	O60602
Protein length	Thr661-Ser858
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	50.20 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	TIL3, TLR5, Toll/interleukin-1 receptor-like protein 3, Toll-like receptor 5
Background	Toll-like receptor 5 (CD285/TLR5) is a ~97 kDa protein. Pattern recognition receptor (PRR) located on the cell surface that participates in the activation of innate immunity and inflammatory response. Recognizes small molecular motifs named pathogen-associated molecular pattern (PAMPs) expressed by pathogens and microbe-associated molecular patterns (MAMPs) usually expressed by resident microbiota. Upon ligand binding such as bacterial flagellins, recruits intracellular adapter proteins MYD88 and TRIF leading to NF-kappa-B activation, cytokine secretion and induction of the inflammatory response. Plays thereby an important role in the relationship between the intestinal epithelium and enteric microbes and contributes to the gut microbiota composition throughout life. 1. Hayashi, F. et al. (2001) Nature 410, 1099-103. PMID: 11323673 2. Blohmke, CJ. et al. (2008) Journal of immunology (Baltimore, Md. : 1950) 180, 7764-73. PMID: 18490781 3. Bielaszewska, M. et al. (2018) International journal of medical microbiology : IJMM 308, 882-889. PMID: 29934223 4. Gewirtz, AT. et al. (2001) Journal of immunology (Baltimore, Md. : 1950) 167, 1882-5. PMID: 11489966 5. Choi, YJ. et al. (2010) The Journal of biological chemistry 285, 37570-8. PMID: 20855887
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human CD285/TLR5 Protein, N-GST

References

Datasheet

Human CD285/TLR5 Recombinant Protein (N-GST).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Human CD285/TLR5 Recombinant Protein (N-GST) (HT266022)

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