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Anti-Human VISTA/B7-H5/PD-1H Recombinant Antibody (SAA0172), APC (HP543137)

Anti-Human VISTA/B7-H5/PD-1H Recombinant Antibody (SAA0172), APC
Anti-Human VISTA/B7-H5/PD-1H Recombinant Antibody (SAA0172), APC
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Vista general
Número de catálogoHP543137
Reactividad de especiesHuman
AplicacionesFCM
Especie huéspedHuman
ClonalidadMonoclonal
IsotipoIgG1, kappa
ID de clonSAA0172
ConjugaciónAPC
Aplicaciones probadas FCM
Objetivo V-set domain-containing immunoregulatory receptor, C10orf54, VSIR, VISTA, SISP1, Stress-induced secreted protein-1, Platelet receptor Gi24, V-set immunoregulatory receptor, Sisp-1, V-type immunoglobulin domain-containing suppressor of T-cell activation
Nivel de endotoxinas Please contact with the lab for this information.
Productos asociados Human IgG1 Recombinant Isotype Control Antibody (HyHEL-10), APC
Número de acceso Q9H7M9
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Estabilidad y almacenamiento Store at 4°C for 12 months. Protect from light. Do not freeze.
Fondo

V-type immunoglobulin domain-containing suppressor of T-cell activation (VSIR/VISTA) is a ~33 kDa protein. Immunoregulatory receptor which inhibits the T-cell response. May promote differentiation of embryonic stem cells, by inhibiting BMP4 signaling. May stimulate MMP14-mediated MMP2 activation.

1. Lines, JL. et al. (2014) Cancer research 74, 1924-32. PMID: 24691993
2. Sakr, MA. et al. (2010) Cancer science 101, 2368-74. PMID: 20666777
Nota For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.
Imágenes
  • Anti-Human VISTA/B7-H5/PD-1H Recombinant Antibody (SAA0172), APC

    Flow cytometry

    Flow-cytometry APC using anti-human VSIR antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an APC anti-human VSIR monoclonal antibody (Catalog HP543137) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

Referencias
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
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Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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