Anti-Human VISTA/B7-H5/PD-1H Reference Antibody (CI-8993, RUO) (HP543026)

Name: Anti-Human VISTA/B7-H5/PD-1H Reference Antibody (CI-8993, RUO)
Brand: abinScience
SKU: HP543026
Price: 176 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.	HP543026
Description	Anti-Human VSIR/VISTA Antibody (CI-8993) (HP543026) is a research-grade recombinant antibody targeting V-set domain-containing immunoregulatory receptor. Produced in mammalian cells with native-like glycosylation. Highlights ●Research Grade — For PK/PD studies, assay development, and ADA research. ●Native Glycosylation — Mammalian expression ensures native-like patterns.
Species reactivity	Human
Applications	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Host species	Human
Isotype	IgG1, kappa
Clone ID	CI-8993
Expression system	Mammalian cells
Clonality	Monoclonal
Target	V-set domain-containing immunoregulatory receptor, C10orf54, VSIR, VISTA, SISP1, Stress-induced secreted protein-1, Platelet receptor Gi24, V-set immunoregulatory receptor, Sisp-1, V-type immunoglobulin domain-containing suppressor of T-cell activation
Endotoxin level	Please contact the lab for this information.
Purity	>95% purity as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	Q9H7M9
Form	Liquid
Storage buffer	0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	VSTB112
Background	V-type immunoglobulin domain-containing suppressor of T-cell activation (VSIR/VISTA) is a ~33 kDa protein. Immunoregulatory receptor which inhibits the T-cell response. May promote differentiation of embryonic stem cells, by inhibiting BMP4 signaling. May stimulate MMP14-mediated MMP2 activation. 1. Lines, JL. et al. (2014) Cancer research 74, 1924-32. PMID: 24691993 2. Sakr, MA. et al. (2010) Cancer science 101, 2368-74. PMID: 20666777
Note	For research use only. Not suitable for clinical or therapeutic use.

Images

Bioactivity

Detects Human VSIR/VISTA in indirect ELISAs.
SDS-PAGE

SDS-PAGE for Research Grade Anti-Human VSIR/VISTA Antibody (CI-8993).
Flow cytometry

Flow-cytometry APC using anti-human VSIR antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an APC anti-human VSIR monoclonal antibody (Catalog HP543026) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow cytometry

Flow-cytometry PE using anti-human VSIR antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an PE anti-human VSIR monoclonal antibody (Catalog HP543026) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow cytometry

Flow-cytometry FITC using anti-human VSIR antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an FITC anti-human VSIR monoclonal antibody (Catalog HP543026) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.