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Anti-Human VISTA/B7-H5/PD-1H Recombinant Antibody (SAA0172), APC (HP543137)

Anti-Human VISTA/B7-H5/PD-1H Recombinant Antibody (SAA0172), APC
Anti-Human VISTA/B7-H5/PD-1H Recombinant Antibody (SAA0172), APC
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  • 100T
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Aperçu
Numéro de catalogueHP543137
Reactivité des espècesHuman
ApplicationsFCM
Espèce hôteHuman
ClonalitéMonoclonal
IsotypeIgG1, kappa
ID de cloneSAA0172
ConjugaisonAPC
Applications testées FCM
Cible V-set domain-containing immunoregulatory receptor, C10orf54, VSIR, VISTA, SISP1, Stress-induced secreted protein-1, Platelet receptor Gi24, V-set immunoregulatory receptor, Sisp-1, V-type immunoglobulin domain-containing suppressor of T-cell activation
Niveau d'endotoxines Please contact with the lab for this information.
Produits associés Human IgG1 Recombinant Isotype Control Antibody (HyHEL-10), APC
Numéro d'accès Q9H7M9
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Stabilité et stockage Store at 4°C for 12 months. Protect from light. Do not freeze.
Contexte

V-type immunoglobulin domain-containing suppressor of T-cell activation (VSIR/VISTA) is a ~33 kDa protein. Immunoregulatory receptor which inhibits the T-cell response. May promote differentiation of embryonic stem cells, by inhibiting BMP4 signaling. May stimulate MMP14-mediated MMP2 activation.

1. Lines, JL. et al. (2014) Cancer research 74, 1924-32. PMID: 24691993
2. Sakr, MA. et al. (2010) Cancer science 101, 2368-74. PMID: 20666777
Note For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.
Images
  • Anti-Human VISTA/B7-H5/PD-1H Recombinant Antibody (SAA0172), APC

    Flow cytometry

    Flow-cytometry APC using anti-human VSIR antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an APC anti-human VSIR monoclonal antibody (Catalog HP543137) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

Reférences
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
Recommandation

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