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Anti-Mycobacterium tuberculosis Isocitrate icl1/Isocitrate lyase 1 Polyclonal Antibody (JN930024)

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Vista general
Número de catálogoJN930024
Descripción
Anti-Mycobacterium tuberculosis Isocitrate lyase/ICL Polyclonal Antibody (JN930024) is a rabbit polyclonal antibody detecting Isocitrate lyase. Suitable for Mycobacterium tuberculosis and Mycobacterium bovis.
Highlights
  • ●Multi-Species — Cross-reactive for translational research.
Reactividad de especiesMycobacterium tuberculosis, Mycobacterium bovis
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Mycobacterium tuberculosis Isocitrate lyase/ICL (Met1-His428).
Objetivo EC:4.1.3.1, ICL, Isocitrase, Isocitratase, Isocitrate lyase, icl
Nivel de endotoxinas Please contact with the lab for this information.
Purificación Purified by antigen affinity column.
Número de acceso P9WKK7
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Fondo

Isocitrate lyase (ICL) is a ~47 kDa protein. Involved in the persistence and virulence of M.tuberculosis. Catalyzes the reversible formation of succinate and glyoxylate from isocitrate, a key step of the glyoxylate cycle, which operates as an anaplerotic route for replenishing the tricarboxylic acid cycle during growth on fatty acid substrates. It could also catalyze the formation of pyruvate and succinate from 2-methylisocitrate, a key step in the methylcitrate cycle (propionate degradation route).

1. Sharma, V. et al. (2000) Nature structural biology 7, 663-8. PMID: 10932251
2. McKinney, JD. et al. (2000) Nature 406, 735-8. PMID: 10963599
3. Kumar, R. et al. (2008) Proteins 72, 892-900. PMID: 18275086
4. Moynihan, MM. et al. (2014) Biochemistry 53, 178-87. PMID: 24354272
Nota For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Mycobacterium tuberculosis Isocitrate icl1/Isocitrate lyase 1 Polyclonal Antibody

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