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Anti-Poliovirus Capsid protein VP3 Recombinant Nanobody (7A) (VK557013)

Anti-Poliovirus Capsid protein VP3 Recombinant Nanobody (7A)
Anti-Poliovirus Capsid protein VP3 Recombinant Nanobody (7A)
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  • 1mg
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Vista general
Número de catálogoVK557013
Descripción
Anti-Poliovirus Capsid protein VP3 Nanobody (7A) [7A] (VK557013) is a alpaca monoclonal antibody detecting Capsid protein VP3 in ELISA, Neutralization.
Reactividad de especiesPoliovirus type 1 (PV1)
AplicacionesELISA, Neutralization
Especie huéspedAlpaca
ClonalidadMonoclonal
IsotipoVHH-8His-Cys-tag
ID de clon7A
Objetivo Genome polyprotein, Capsid protein VP3, Virion protein 3
Nivel de endotoxinas Please contact with the lab for this information.
Pureza >95% as determined by SDS-PAGE.
Purificación Purified by Nickel column.
Número de acceso P03300
Forma Liquid
Buffer de almacenamiento 0.01M PBS pH 7.4

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto
Aplicación Dilución
ELISA 1:2000-1:20000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Genome polyprotein (Capsid protein VP3) is a ~246 kDa protein. Forms an icosahedral capsid of pseudo T=3 symmetry with capsid proteins VP2 and VP3. The capsid is 300 Angstroms in diameter, composed of 60 copies of each capsid protein and enclosing the viral positive strand RNA genome. Capsid protein VP1 mainly forms the vertices of the capsid. Capsid protein VP1 interacts with host cell receptor PVR to provide virion attachment to target host epithelial cells. This attachment induces virion internalization predominantly through clathrin- and caveolin-independent endocytosis in Hela cells and through caveolin-mediated endocytosis in brain microvascular endothelial cells.

1. Hogle, JM. et al. (1985) Science (New York, N.Y.) 229, 1358-65. PMID: 2994218
2. Strauss, M. et al. (2013) Journal of virology 87, 3903-14. PMID: 23365424
3. Strauss, M. et al. (2015) Journal of virology 89, 4143-57. PMID: 25631086
4. Brandenburg, B. et al. (2007) PLoS biology 5, e183. PMID: 17622193
5. Coyne, CB. et al. (2007) The EMBO journal 26, 4016-28. PMID: 17717529
6. Bergelson, JM. (2008) Trends in microbiology 16, 44-7. PMID: 18191571
Nota For research use only.
Imágenes
  • Anti-Poliovirus Capsid protein VP3 Recombinant Nanobody (7A)

    SDS-PAGE

    SDS-PAGE for Anti-Poliovirus Capsid protein VP3 Nanobody (7A)

Referencias
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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