Anti-Poliovirus type 1 VP1/Capsid protein VP1 Polyclonal Antibody (VK674014)

Name: Anti-Poliovirus type 1 VP1/Capsid protein VP1 Polyclonal Antibody
Brand: abinScience
SKU: VK674014
Price: 168 USD
Availability: InStock

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Spec:

50ug
100ug

Number:

Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

VK674014

Species reactivity

Poliovirus type 1 (strain Mahoney)

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Poliovirus type 1 VP1/Capsid protein VP1 (Gly580-Tyr881).

Target

Genome polyprotein, P1, Capsid protein VP1

Purification

Purified by antigen affinity column.

Accession

P03300

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Genome polyprotein (VP1/Capsid protein VP1) is a ~246 kDa protein. Forms an icosahedral capsid of pseudo T=3 symmetry with capsid proteins VP2 and VP3. The capsid is 300 Angstroms in diameter, composed of 60 copies of each capsid protein and enclosing the viral positive strand RNA genome. Capsid protein VP1 mainly forms the vertices of the capsid. Capsid protein VP1 interacts with host cell receptor PVR to provide virion attachment to target host epithelial cells. This attachment induces virion internalization predominantly through clathrin- and caveolin-independent endocytosis in Hela cells and through caveolin-mediated endocytosis in brain microvascular endothelial cells.

1. Hogle, JM. et al. (1985) Science (New York, N.Y.) 229, 1358-65. PMID: 2994218

2. Strauss, M. et al. (2013) Journal of virology 87, 3903-14. PMID: 23365424

3. Strauss, M. et al. (2015) Journal of virology 89, 4143-57. PMID: 25631086

4. Brandenburg, B. et al. (2007) PLoS biology 5, e183. PMID: 17622193

5. Coyne, CB. et al. (2007) The EMBO journal 26, 4016-28. PMID: 17717529

6. Bergelson, JM. (2008) Trends in microbiology 16, 44-7. PMID: 18191571

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-Poliovirus type 1 VP1/Capsid protein VP1 polyclonal antibody at 1ug/mL on recombinant Poliovirus type 1 VP1/Capsid protein VP1 (Catalog No. VK674012).

References

Datasheet