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Anti-Poliovirus Capsid protein VP3 Recombinant Nanobody (7A) (VK557013)

Anti-Poliovirus Capsid protein VP3 Recombinant Nanobody (7A)
Anti-Poliovirus Capsid protein VP3 Recombinant Nanobody (7A)
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Aperçu
Numéro de catalogueVK557013
Description
Anti-Poliovirus Capsid protein VP3 Nanobody (7A) [7A] (VK557013) is a alpaca monoclonal antibody detecting Capsid protein VP3 in ELISA, Neutralization.
Reactivité des espècesPoliovirus type 1 (PV1)
ApplicationsELISA, Neutralization
Espèce hôteAlpaca
ClonalitéMonoclonal
IsotypeVHH-8His-Cys-tag
ID de clone7A
Cible Genome polyprotein, Capsid protein VP3, Virion protein 3
Niveau d'endotoxines Please contact with the lab for this information.
Pureté >95% as determined by SDS-PAGE.
Purification Purified by Nickel column.
Numéro d'accès P03300
Forme Liquid
Tampon de stockage 0.01M PBS pH 7.4

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit
Application Dilution
ELISA 1:2000-1:20000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Genome polyprotein (Capsid protein VP3) is a ~246 kDa protein. Forms an icosahedral capsid of pseudo T=3 symmetry with capsid proteins VP2 and VP3. The capsid is 300 Angstroms in diameter, composed of 60 copies of each capsid protein and enclosing the viral positive strand RNA genome. Capsid protein VP1 mainly forms the vertices of the capsid. Capsid protein VP1 interacts with host cell receptor PVR to provide virion attachment to target host epithelial cells. This attachment induces virion internalization predominantly through clathrin- and caveolin-independent endocytosis in Hela cells and through caveolin-mediated endocytosis in brain microvascular endothelial cells.

1. Hogle, JM. et al. (1985) Science (New York, N.Y.) 229, 1358-65. PMID: 2994218
2. Strauss, M. et al. (2013) Journal of virology 87, 3903-14. PMID: 23365424
3. Strauss, M. et al. (2015) Journal of virology 89, 4143-57. PMID: 25631086
4. Brandenburg, B. et al. (2007) PLoS biology 5, e183. PMID: 17622193
5. Coyne, CB. et al. (2007) The EMBO journal 26, 4016-28. PMID: 17717529
6. Bergelson, JM. (2008) Trends in microbiology 16, 44-7. PMID: 18191571
Note For research use only.
Images
  • Anti-Poliovirus Capsid protein VP3 Recombinant Nanobody (7A)

    SDS-PAGE

    SDS-PAGE for Anti-Poliovirus Capsid protein VP3 Nanobody (7A)

Reférences
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Recommandation

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