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Anti-Poliovirus type 1 VP3/Capsid protein VP3 Polyclonal Antibody (VK644014)

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Vista general
Número de catálogoVK644014
Reactividad de especiesPoliovirus type 1 (PV1)
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Poliovirus type 1 VP3/Capsid protein VP3 (Gly342-Gln579).
Objetivo Genome polyprotein, P1, Capsid protein VP3
Purificación Purified by antigen affinity column.
Número de acceso P03300
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Genome polyprotein (VP3/Capsid protein VP3) is a ~246 kDa protein. Forms an icosahedral capsid of pseudo T=3 symmetry with capsid proteins VP2 and VP3. The capsid is 300 Angstroms in diameter, composed of 60 copies of each capsid protein and enclosing the viral positive strand RNA genome. Capsid protein VP1 mainly forms the vertices of the capsid. Capsid protein VP1 interacts with host cell receptor PVR to provide virion attachment to target host epithelial cells. This attachment induces virion internalization predominantly through clathrin- and caveolin-independent endocytosis in Hela cells and through caveolin-mediated endocytosis in brain microvascular endothelial cells.

1. Hogle, JM. et al. (1985) Science (New York, N.Y.) 229, 1358-65. PMID: 2994218
2. Strauss, M. et al. (2013) Journal of virology 87, 3903-14. PMID: 23365424
3. Strauss, M. et al. (2015) Journal of virology 89, 4143-57. PMID: 25631086
4. Brandenburg, B. et al. (2007) PLoS biology 5, e183. PMID: 17622193
5. Coyne, CB. et al. (2007) The EMBO journal 26, 4016-28. PMID: 17717529
6. Bergelson, JM. (2008) Trends in microbiology 16, 44-7. PMID: 18191571
Nota For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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